Mass Spectrometry Proteomics

Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. Liquid chromatography is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). Multidimensional Protein Identification Technology (MudPIT) is used to analyze the proteomes of organisms. Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Imaging mass spectrometry (IMS) using matrix-assisted laser desorption ionization (MALDI) is a new and effective tool for molecular studies of complex biological samples such as tissue sections.

  • Protein identification
  • De novo (peptide) sequencing
  • Protein quantitation
  • Protein structure determination
  • Proteogenomics

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